Highlights:

Peptide design, synthesis, and conjugation– Selection of the best possible antigenic peptides; synthesis and peptide conjugation to a carrier protein.

Optimized immunisation protocols.

Hybridoma fusion and screening.

Sub-cloning and delivery.

HYBRIDOMA DEVELOPMENT SERVICES

Why choose VAL for your hybridoma development ?

As part of our commitment to the scientific community, we offer a LOW RISK and guaranteed Monoclonal Antibody development package for the following applications:

  • WB
  • IHC
  • ELISA
  • IF
  • SANDWICH ELISA
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VAL hybridoma development service content

  • ANTIGEN DESGIN: Using bioinformatics, VAL will assist with the selection of the peptide antigen, identifying exposed, hydrophilic, flexible and immunogenic sites.
  • PEPTIDE SYNTHESIS & CONJUGATION: Peptide synthesis (at least 80% purity, quality control by HPLC, MS) and conjugation to the desired protein carriers.
  • IMMUNISATION: Standard immunization according to a schedule over 42 days.
  • CELL FUSION: Splenocytes with best serum antibody response are fused with myeloma cell line.
  • HYBRIDOMA SELECTION AND SCREENING: Hybrid cells selection (HAT) and culture supernatant screening vs. target antigen.
  • EXPASION OF BEST CLONES:
    • Hybridoma cell lines subcloned to monoclonality & tested in the selected assay systems
    • Final antibodies selected and characterised.
    • Clones are (i) supplied to sponsor (ii) frozen and banked at VAL premises as back-up. Thereafter the sponsor may elect to have antibody produced/purified by VAL.
Click here to ask us for a quote on our custom antibody services