- Immunogenicity profiling of SARS-CoV-2 identified N, M, ORF3a, ORF7a as most antigenic viral proteins.
- Immunodominant epitopes were identified using EpitopePredikt, then selected epitopes fused together and expressed on EpitoGen® scaffold.
- Prevalent mutants were included to enhance the detection accuracy of the non-spike complex. Some mutations will create new epitopes to which specific antibody response is elicited. We included 29 prevalent mutations into the EpitoGen® Differential to enhance sensitivity.
-Nucleocapsid (N6m) mutations: S33I, A35V, P46S, D103Y, A152S, A156S, P168S, G125V, Q229H, M234I, S235F, T247I, A251V, T366I, K373N, D377Y, P383S, T391I, D401Y.
–Membrane mutations: D3G, K15R, D209Y.
–ORF3a mutations: K136E, A143S, Q213K, T223I
-ORF7a mutations: H73Y, R80I, P84S